Journal: Cancers

Article Title: CSF1R Ligands Expressed by Murine Gliomas Promote M-MDSCs to Suppress CD8 + T Cells in a NOS-Dependent Manner

doi: 10.3390/cancers16173055

Figure Lengend Snippet: Expression of CSF ligands in murine glioma and human GBM: ( A ) Murine KR158B gliomas express the CSF ligands M-CSF and IL-34 but not GM-CSF. KR158B cells were plated at different numbers (50, 100, 200 thousand) in 24 well plates and the conditioned media were subjected to ELISA after 24 h for cytokine quantification. CSF1R ligands, M-CSF and IL-34, were found at a higher concentration in the media compared to GM-CSF (n = 3 experiments). ( B ) Human GBM expression of CSF ligands was determined from a search of the OncoDB database. TCGA dataset was queried for GBM gene expression. Results were compared to normal brain tissue from GTEx. CSF-1 (M-CSF) was differentially upregulated in the GBM microenvironment (n = 148 patients) compared to normal brain (n = 200 patients). CSF-1 was found at the highest level compared to the other CSF ligands. ( C ) Single-Cell Portal database from Broad Institute was accessed for evaluation of CSF ligand expression. The “Programs, Origins, and Niches of Immunomodulatory Myeloid Cells in Human Gliomas” dataset was used for analysis. Multiple human tumors (85) and cells (515,782) were analyzed. CSF-1 and IL34 are expressed by the malignant cell population. CSF-2 and CSF-3 (GM-CSF and G-CSF) were lowly expressed. Students’ t -test statistical analysis was conducted. Differences are compared to the stimulated control condition. p -values: >0.05 (ns), 0.0332 (*), 0.0002 (***), <0.0001 (****).

Article Snippet: Single Cell Portal database from Broad Institute was accessed for evaluation of CSF ligand expression.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay, Gene Expression, Control

Journal: Kidney Diseases

Article Title: Localization of Cell Receptor-Related Genes of SARS-CoV-2 in the Kidney through Single-Cell Transcriptome Analysis

doi: 10.1159/000508162

Figure Lengend Snippet: Expression of genes associated with SARS-CoV-2 entry in single-cell transcriptome sequencing database. a UMAP visualization displaying major cell clusters (22,268 single cells). b Dot plot of LRP2 and CUBN (marker genes of PT cells), ACE2, TMPRSS2, and SLC6A19; dot size represents the fraction of cells expressing a cell type, and color intensity binned count-based expression level amongst expressing cells. c UMAP projection, points colored by detection of ACE2 (left), TMPRSS2 (middle), and SLC6A19 (right). Blue, RNA positive; gray, RNA negative. d Volcano plot displaying differential expression of genes between ACE2+ and ACE2– PT cells. Red, up-regulated genes; blue, down-regulated genes. e Bar plot presenting significantly enriched GO terms obtained from GO enrichment analysis performed with differential expression of genes between ACE2+ and ACE2– PT cells.

Article Snippet: In the latest human kidney single-cell transcriptome database (scRNA-seq) from the Broad Institute and the University of Michigan [ ], we found significant expression of ACE2 in PT cells, glomerular parietal epithelial cells, and descending thin limb cells (Fig. ; online suppl.

Techniques: Expressing, Sequencing, Marker, Quantitative Proteomics

Journal: Kidney Diseases

Article Title: Localization of Cell Receptor-Related Genes of SARS-CoV-2 in the Kidney through Single-Cell Transcriptome Analysis

doi: 10.1159/000508162

Figure Lengend Snippet: Expression of genes associated with SARS-CoV-2 entry in single-nuclear transcriptome. a Dot plot of LRP2 and CUBN (marker genes of PT cells), ACE2, TMPRSS2, and SLC6A19; dot size represents the fraction of cells expressing each cell type, and color intensity binned count-based expression level amongst expressing cells. b Bar plot presenting significantly enriched GO terms obtained from GO enrichment analysis performed with differential expression of genes between ACE2+ and ACE2– PT cells. c Violin plot showing ACE2, SLC6A19, and TMPRSS2 expression distribution among different cell clusters.

Article Snippet: In the latest human kidney single-cell transcriptome database (scRNA-seq) from the Broad Institute and the University of Michigan [ ], we found significant expression of ACE2 in PT cells, glomerular parietal epithelial cells, and descending thin limb cells (Fig. ; online suppl.

Techniques: Expressing, Marker, Quantitative Proteomics

Journal: Kidney Diseases

Article Title: Localization of Cell Receptor-Related Genes of SARS-CoV-2 in the Kidney through Single-Cell Transcriptome Analysis

doi: 10.1159/000508162

Figure Lengend Snippet: Expression of genes associated with SARS-CoV-2 entry in a single-cell transcriptome sequencing database of Asians. a Dot plot of LRP2 and CUBN (marker genes of PT cells), ACE2, TMPRSS2, and SLC6A19; dot size represents the fraction of cells expressing each cell type, and color intensity binned count-based expression level amongst expressing cells. b Bar plot presenting significantly enriched GO terms obtained from GO enrichment analysis performed with differential expression of genes between ACE2+ and ACE2– PT cells.

Article Snippet: In the latest human kidney single-cell transcriptome database (scRNA-seq) from the Broad Institute and the University of Michigan [ ], we found significant expression of ACE2 in PT cells, glomerular parietal epithelial cells, and descending thin limb cells (Fig. ; online suppl.

Techniques: Expressing, Sequencing, Marker, Quantitative Proteomics